The Microbial Limits Test (or Microbial Enumeration Test) is designed to perform the qualitative and quantitative estimations of specific viable microorganisms present in samples. It includes tests for total viable count (bacteria and fungi) and screening for pathogenic organisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella, Clostridium, and Escherichia coli. The type of pathogen screening will depend on the product (e.g., oral products are typically tested for absence of E. coli and Salmonella). When test samples have antimicrobial activity or when they include antimicrobial substances, these antimicrobial properties are eliminated by dilution, filtration, neutralization, inactivation, or other appropriate means.
Samples should be submitted at a temperature similar to that in which the sample is stored. Avoid extreme temperatures.
|Assay Description||Service Code||Volume|
|Microbial Limits Test (USP <61> and <62> or EP) Total aerobic count and selective organisms
(example: E. coli, Pseudomonas, Salmonella and Staph. aureus)
|MLT01||> 20g or mL|
|Microbial Limits pathogen screening per organism (USP <61>) with no plate count||MLT02||> 10g or mL|
|Microbial Limits for nutritional or personal care products
Total aerobic count and selective organisms
(example: E. coli, Salmonella, Pseudomonas aeruginosa, and Staph. aureus)
|MLT03||> 20g or mL|
|Validation of Microbial Recovery (USP Microbial Limits Testing) includes val. of aerobic plate count, fungi count and selective organisms as needed
(example: E. coli, Pseudomonas, Salmonella, and Staphylococcus aureus)
|MLT04||> 100g or mL|
|MLT method development||MLT05||Product specific|
|MLT custom method||MLT06||Product specific|